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PLC γ1 (phospho-Y783) polyclonal antibody（KU）

货号: BS4157

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4157

Product Name :PLC γ1 (phospho-Y783) polyclonal antibody（KU）

Swiss-Prot :P19174

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000

Background :Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. There are many mammalian PLC isozymes, including PLC β1, PLC β2, PLC β3, PLC β4, PLC γ1, PLC γ2, PLC δ1, PLC δ2 and PLC ε. PLC γ1 is widely distributed in bronchiolar epithelium, type I and II pneumocytes and fibroblasts of the interstitial tissue. Actinregulatory protein Villin is tyrosine phosphorylated and associates with PLC γ1 in the brush border of intestinal epithelial cells. Villin regulates PLC γ1 activity by modifying its own ability to bind phosphatidylinositol 4,5-biphosphate. PLC γ1 binds α1β1 Integrin and modulates α1β1 Integrin-specific adhesion. PLC γ1 and Ca2+ play a direct role in VEGF-regulated endothelial growth, however this signaling pathway is not linked to FGF-mediated effects in primary endothelial cells.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-PLC γ1 (Y783) polyclonal antibody detects endogenous levels of PLC γ1 protein only when phosphorylated at Tyr783

BiowMW :~ 148 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1; PLC-148; PLC148; Phosphoinositide phospholipase C-gamma-1; Phospholipase C-II; PLC-II; Phospholipase C2; PLC2I; Phospholipase C-gamma-1; PLC-gamma-1; PLCG1; PLC1; PLCγ1;

Immunogen :Synthetic phosphopeptide derived from human PLC γ1 around the phosphorylation site of Tyrosine 783.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-PLC γ1 (Y783) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with UV for 5 minutes then repair for 4 hours whole cell lysate(40ug) Lane3:HEK293T treated with UV for 5 minutes then repair for 6 hours whole cell lysate(40ug) Lane4:HEK293T treated with UV for 5 minutes then repair for 16 hours whole cell lysate(40ug) Lane5:HEK293T treated with UV for 5 minutes then repair for 24 hours whole cell lysate(40ug) Lane6:C6 whole cell lysate(40ug) Lane7:The Kidney tissue lysate of Mouse(40ug)

Immunohistochemistry (IHC) analyzes of p-PLCG1 (Y783) pAb in paraffin-embedded human breast carcinoma tissue.

Ginsenoside Rg1 attenuates LPS-induced cognitive impairments and neuroinflammation by inhibiting NOX2 and Ca2+–CN–NFAT1 signaling in mice

JournalJournal of Functional Foods

ApplicationWB

IF4.451

SpeciesMouse

PMID
Chronic glucocorticoid exposure accelerates Aβ generation and neurotoxicity by activating calcium-mediated CN-NFAT1 signaling in hippocampal neurons in APP/PS1 mice

JournalFOOD AND CHEMICAL TOXICOLOGY

ApplicationWB

IF5.572

SpeciesMouse

PMID36075474