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PDPK1 (phospho-S241) polyclonal antibody（KU）

货号: BS4155

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4155

Product Name :PDPK1 (phospho-S241) polyclonal antibody（KU）

Swiss-Prot :O15530

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :PDK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of hosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDK plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-PDPK1 (S241) polyclonal antibody detects endogenous levels of PDPK1 protein when phosphorylated at Ser241.

BiowMW :~ 63 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Phosphoinosotode Signaling,Protein Kinase C Signaling,Translational Contral elF4 and p70 S6 Kinase,Inhibition of Apoptosis,Insulin &Glucose Signaling,

Alternative Name :3-phosphoinositide-dependent protein kinase 1; hPDK1; PDPK1; PDK1

Immunogen :Synthetic phosphopeptide derived from human PDPK1 around the phosphorylation site of Serine 241.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-PDK1 (S241) pAb at 1:1000 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with EGF(100ng/ml,30 minutes) whole cell lysate(40ug) Lane3:C6 whole cell lysate(40ug) Lane4:CT26 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of p-PDPK1 (S241) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.