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NFkB-p65 (phospho-S529) polyclonal antibody

货号: BS4137

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4137

Product Name :NFkB-p65 (phospho-S529) polyclonal antibody

Swiss-Prot :Q04206

Host :Rabbit

Reactivity :Human

Applications :WB IHC IP

Application_all :WB: 1:500~1:1000 IHC: 1:100~1:200 IP: 1:50~1:200

Background :p65 is a subunit of the nuclear factor kappa B. The transcription factor NFkB is widely recognized as a critical mediator of immune and inflammatory responses. In most cell types, NFkB is found in the cytoplasm where it is associated with an inhibitory prd in many tissues. A high proportion of spontaneous NIH/3T3 transformants overexpress c-Met and by transfection analysis the c-Met proto-oncogene has been shown to exhibittransforming activity.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-NFkB-p65 (S529) polyclonal antibody detects endogenous levels of NFkB-p65 protein only when phosphorylated at Ser529

BiowMW :~ 75 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :NF-kB Signaling,NF-kB Signaling,Immune Cell Signaling,Signaling Pathways Activating p38 MAP KinaseSTING Signaling,T cell receptor signaling,Inhibition of Apoptosis,Contribution of Soluble Factors to EMT,

Alternative Name :p65; p65 NF kappaB; p65 NFkB; RELA; NFKB P65; Transcription Factor p65; Nuclear factor NF-kappa-B p65 subunit; NFkB-p65; NFKB3

Immunogen :Synthetic phosphopeptide derived from human NFkB-p65 around the phosphorylation site of Serine 529.

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of NF-kappaB p65 (pS529) expression in A2780 (A), A549 (B) whole cell lysates.

Immunohistochemical analysis of NF-kappaB p65 (pS529) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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