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NFkB-p100 (phospho-S865) polyclonal antibody

货号: BS4129

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4129

Product Name :NFkB-p100 (phospho-S865) polyclonal antibody

Swiss-Prot :Q00653

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :The NFκB transcription factor was originally identified as a protein complex consisting of a DNA binding subunit and an associated protein. The subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino-terminus of a precursor designated p105. A cDNA has been isolated that encodes an alternative DNA binding subunit of NFκB. It is synthesized as a protein that is expressed in a variety of cell types and, like p105, undergoes cleavage to generate its NFκB subunit, in this case a protein designated p52 (previously referred to as p49). In contrast to p50 derived from p105, p52 acts in synergy with p65 to stimulate the HIV enhancer in transiently transfected Jurkat cells.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-NFkB-p100 (S865) polyclonal antibody pAb detects endogenous levels of NFkB-p100 protein only when phosphorylated at Ser865.

BiowMW :~ 100, 120 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :NF-kB Signaling,STING Signaling,Inhibition of Apoptosis,

Alternative Name :Nuclear factor NF-kappa-B p100 subunit; DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10 protein; Nuclear factor of kappa light; polypeptide gene enhancer in B-cells 2; Oncogene Lyt-10; Lyt10; NFKB2; LYT10

Immunogen :Synthetic phosphopeptide derived from human NFkB-p100 around the phosphorylation site of Serine 865.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of NFkB-p100 (phospho-S865) polyclonal antibody at 1:500 dilution Lane1:NIH-3T3 whole cell lysate(40ug) Lane2:NIH-3T3(PMA, 60ng/ml, 5min)whole cell lysate(40ug) Lane3:NIH-3T3(PMA, 60ng/ml, 15min)whole cell lysate(40ug) Lane4:NIH-3T3(PMA, 60ng/ml, 30min)whole cell lysate(40ug) Lane5:NIH-3T3(EGF, 0.1ng/ml, 30min)whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of p-NFkB-p100 (S865) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

The potential mechanism of Isodon suzhouensis against COVID-19 via EGFR/TLR4 pathways

Journal10.26599/FSHW.2023.9250011

ApplicationWB

IF5.6

SpeciesMouse,Rat

PMID