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MEK2 (phospho-T394) polyclonal antibody（KU）

货号: BS4122

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4122

Product Name :MEK2 (phospho-T394) polyclonal antibody（KU）

Swiss-Prot :P36507

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IP

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :MEK1 (Mitogen activated protein kinase kinase 1) catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. MEK1 activates ERK1 and ERK2 MAP kinases. Mitogen activated protein kinase kinase 2 (MEK2 or MAPKK2) is a member of a family of tyrosine/threonine protein kinases that activate the ERK1 and 2 and MAPK enzymes by phosphorylating both residues within the threonine/glutamate/tyrosine (TEY) motif in the activation loop. MEK1 and 2 are also activated by dual phosphorylation, which occurs on serine 218 and 222, in the activation loop of the MEK. Threonine 292 of MEK1 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK1.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-MEK2 (T394) polyclonal antibody detects endogenous levels of MEK2 protein only when phosphorylated at Thr394

BiowMW :~ 45 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Warburg Effect,G Protein-coupled Receptors Signaling to MAPK Erk,

Alternative Name :Dual specificity mitogen-activated protein kinase kinase 2; MAP kinase kinase 2; MAPKK 2; ERK activator kinase 2; MAPK/ERK kinase 2; MEK 2; MAP2K2; MEK-2; MKK2; PRKMK2

Immunogen :Synthetic phosphopeptide derived from human MEK2 around the phosphorylation site of Threonine 394.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-MEK2 (T394) pAb at 1:2000 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with UV for 5 minutes then repair for 16 hours whole cell lysate(40ug) Lane3:The Kidney tissue lysate of Rat(40ug) Lane4:The Kidney tissue lysate of Mouse(40ug)

Immunohistochemistry (IHC) analyzes of p-MEK 2(T394) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.