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Phospho-JAK2-Y1007/1008 polyclonal antibody

货号: BS4109

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4109

Product Name :Phospho-JAK2-Y1007/1008 polyclonal antibody

Swiss-Prot :O60674

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC IF IP

Application_all :WB,1:500 - 1:1000|IHC,1:50 - 1:200|IF: 1:100 - 1:500|IP: 1:50~1:200

Background :This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated Antibodies

BiowMW :131KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :JAK2;JTK10;THCYT3

Immunogen :A synthetic phosphorylated peptide around Y1007 & Y1008 of human JAK2(NP_004963.1?).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot (WB) analysis of Phospho-JAK2-Y1007/1008 polyclonal antibody at 1:1000 dilution Lane1:K562 treated with GM-CSF(100 ng/ml) at 37℃ for 15 minutes whole cell lysate(30ug) Lane2:K562 whole cell lysate(30ug)

Immunohistochemistry of paraffin-embedded human liver cancer using Phospho-JAK2-Y1007/1008 antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of K562 cells using Phospho-JAK2-Y1007/1008 pAb at dilution of 1:200 (40x lens).

Immunoprecipitation of K562 cell lysates using Phospho-JAK2-Y1007/1008 pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using Phospho-JAK2-Y1007/1008 pAb.

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