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IRS-1 (Phospho-Ser307) polyclonal antibody（KU）

货号: BS4104

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4104

Product Name :IRS-1 (Phospho-Ser307) polyclonal antibody（KU）

Swiss-Prot :P35568

Host :Rabbit

Reactivity :Human

Applications :WB

Application_all :WB: 1:1000~1:2000

Background :Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase. IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin. IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK and IKK while Ser789 is phosphorylated by SIK-2, a member of the AMPK family. The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively. Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :IRS-1 (Phospho-Ser307) polyclonal antibody detects endogenous levels of IRS-1 protein only when phosphorylated at Ser307.

BiowMW :~ 160 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Translational Contral elF4 and p70 S6 Kinase,Insulin &Glucose Signaling,

Alternative Name :Insulin receptor substrate 1; IRS-1; IRS1

Immunogen :Synthetic phosphopeptide derived from human IRS-1 around the phosphorylation site of Serine 307.

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot (WB) analysis of IRS-1 (Phospho-Ser307) polyclonal antibody at 1:1000 dilution Lane1:MCF-7 treated with λ-phosphatase whole cell lysate(30ug) Lane2:MCF-7 whole cell lysate(30ug)

Puerarin attenuates endothelial insulin resistance through inhibition of inflammatory response in an IKKβ/IRS-1-dependent manner

JournalBiochimie

ApplicationWB

IF3.413

SpeciesHuman

PMID