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CrkL (phospho-Y207) polyclonal antibody（KU）

货号: BS4056

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4056

Product Name :CrkL (phospho-Y207) polyclonal antibody（KU）

Swiss-Prot :P46109

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Crk, is a transformation-specific protein that induces elevation of cellular phosphotyrosine levels, but lacks tyrosine kinase activity itself. A second protein, Nck, consists solely of three SH3 domains and one SH2 domain, while GRB2 contains an SH2 domain flanked on both sides by SH3 domains. A member of this protein class, Crk-L, is encoded by a gene located on chromosome 22, band 11, centromeric of the chronic myelogenous leukemia breakpoint region. Crk-L encodes a 303 amino acid protein with one SH2 and two SH3 domains, and a predicted molecular weight of 36 kDa.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-Crk-L (Y207) polyclonal antibody detects endogenous levels of Crk-L protein only when phosphorylated at Tyr207.

BiowMW :~ 39 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Crk L; Crk like protein; Crk-like protein; Crkl; Crkol; Oncogene CrkL;

Immunogen :Synthetic phosphopeptide derived from human CrkL around the phosphorylation site of Tyrosine 207.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-CrkL (Y207) pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:PMVEC whole cell lysate(40ug) Lane4:CT26 whole cell lysate(40ug) Lane5:SGC7901 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of p-Crk-L (Y207) pAb in paraffin-embedded human colon cancer tissue.

ABL SH3 mutant inhibits BCR-ABL activity and increases imatinib sensitivity by targeting RIN1 protein in CML cell

JournalCancer Letters

ApplicationWB

IF8.679

SpeciesHuman

PMID26321052