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Crk2 (phospho-Y221) polyclonal antibody（KU）

货号: BS4055

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4055

Product Name :Crk2 (phospho-Y221) polyclonal antibody（KU）

Swiss-Prot :P46108

Host :Rabbit

Reactivity :Human

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Crk I and Crk II are encoded by the same gene, which undergoes alternative splicing to yield these two proteins, but differ in their biological activities. Crk II has less transforming activity than Crk I, although both Crk I and Crk II bind to many tyrosine-phosphorylated proteins that bind to GRB2. In addition, Crk II becomes rapidly tyrosine-phosphorylated in response to stimulation with insulin-like growth factor I (IGF-I) and might be involved in the IGF-I receptor signalling pathway.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Crk2 (phospho-Y221) polyclonal antibody detects endogenous levels of Crk2 protein only when phosphorylated at Tyr221.

BiowMW :~ 34 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Adapter molecule crk; Proto-oncogene c-Crk; p38 CRK; CRK; CRKII

Immunogen :Synthetic phosphopeptide derived from human Crk2 around the phosphorylation site of Tyrosine 221.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of Crk2 (phospho-Y221) pAb at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:Hela whole cell lysate(40ug) Lane3:MCF-7 whole cell lysate(40ug) Lane4:The Kidney tissue lysate of Rat(40ug) Lane5:The Kidney tissue lysate of Mouse(40ug)