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CREB (phospho-S133) polyclonal antibody

货号: BS4053

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS4053

Product Name :CREB (phospho-S133) polyclonal antibody

Swiss-Prot :P16220

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IP

Application_all :WB: 1:500~1:1000 IHC: 1:100~1:200 IP: 1:10~1:100

Background :Cyclic AMP Response Element Binding protein (CREB) is a basic / leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation occurs via p44 / 42 MAP kinase and p90RSK and also via p38 MAP kinase and MSK 1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-CREB (S133) polyclonal antibody detects endogenous levels of CREB-1 protein only when phosphorylated at Ser133.

BiowMW :~ 43 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :ErbB HER Signaling，MAPK Erk in Growth and Differentiation Pathway，Inhibition of Apoptosis，Dopamine Signaling in Parkinson\'s Disease Plathway，Signaling Pathways Activating p38 MAP Kinase，T cell receptor signaling

Alternative Name :Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1; CREB1

Immunogen :Synthetic phosphopeptide derived from human CREB around the phosphorylation site of Serine 133.

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of CREB (Phospho-S133) expression in C6 (A), NIH3T3 (B), HEK293T (C), H1792 (D) whole cell lysates.

Immunohistochemical analysis of CREB (Phospho-S133) staining in human breast cancer formalin fixed paraffin embedded tissue section.

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