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cPLA2 (phospho-S505) polyclonal antibody（KU）

货号: BS4052

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4052

Product Name :cPLA2 (phospho-S505) polyclonal antibody（KU）

Swiss-Prot :P47712

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Phospholipase A2s (PLA2s) constitute a family of esterases that hydrolyze the sn-2-acyl ester bond in glycerophospholipid molecules. These enzymes are generally calcium-dependent and have been found both intra- and extracellularly. By hydrolyzing the sn-2 bond in glycerophospholipids, PLA2s release fatty acids. One such fatty acid, arachidonic acid, generates the substrates for the initiation of the arachidonic acid cascade that produces various eicosanoids (i.e. prostaglandins, leukotrienes and thromboxanes), many of which are potent mediators of inflammation. PLA2s include both the relatively low molecular weight type I and type II enzymes and the form known as cytoplasmic PLA2 (cPLA2). cPLA2 is present in the cytosol of various cells and tissues including platelets, macrophages and monoblasts; and preferentially hydrolyzes the sn-2 position of phospholipid molecules, releasing free arachidonate.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-cPLA2 (S505) polyclonal antibody detects endogenous levels of cPLA2 protein only when phosphorylated at Ser505.

BiowMW :~ 100 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Phospholipase Signaling,G Protein-coupled Receptors Signaling to MAPK Erk,

Alternative Name :Cytosolic phospholipase A2; cPLA2; Phospholipase A2 group IVA; Phospholipase A2; Phosphatidylcholine 2-acylhydrolase Lysophospholipase; PLA2G4A; CPLA2; PLA2G4

Immunogen :Synthetic phosphopeptide derived from human cPLA2 around the phosphorylation site of Serine 505.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-cPLA2 (S505) pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:The Uterus tissue lysate of Rat(40ug) Lane4:The Uterus tissue lysate of Mouse(40ug) Lane5:3T3-L1 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of p-cPLA2 (S505) pAb in paraffin-embedded human breast cancer tissue.

The protective role of oxymatrine on neuronal cell apoptosis in the hemorrhagic rat brain

JournalJournal Of Ethnopharmacology

ApplicationWB

IF4.36

SpeciesRat

PMID22750437
Regulated Hypoxia/Reperfusion-Dependent Modulation of ERK1/2, cPLA2, and Bcl-2/Bax : A Potential Mechanism of Neuroprotective Effect of Penehyclidine Hydrochloride

JournalInternational Journal Of Neuroscience

ApplicationWB

IF2.292

SpeciesRat

PMID21545309
Ruscogenin suppresses mouse neutrophil activation: Involvement of protein kinase A pathway

JournalJournal Of Steroid Biochemistry And Molecular Biology

ApplicationWB

IF3.813

SpeciesMouse

PMID26134424
UCN enhances TGF-beta-mediated mitoinhibition of VSMCs via counteracting TGF-beta-induced cPLA2 expression and activation

JournalInternational Journal Of Biochemistry & Cell Biology

ApplicationWB

IF3.144

SpeciesRat

PMID27702653