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c-Jun (phospho-Y170) polyclonal antibody（KU）

货号: BS4050

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4050

Product Name :c-Jun (phospho-Y170) polyclonal antibody（KU）

Swiss-Prot :P05412

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :The c-Jun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus v-Jun oncogene. The c-Jun protein, along with c-Fos, is a component of the AP-1 transcriptional complex. c-Jun can form either Jun/Jun homodimers or Jun/Fos heterodimers via the leucine repeats in both proteins. Homo- and heterodimers bind to the TGACTCA consensus sequence present in numerous promoters and initially identified as the phorbol ester tumor promoter response element (TRE). Two additional genes, Jun B and Jun D, have been shown to be almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. All three form heterodimers among themselves and with c-Fos and other members of the Fos gene family.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-c-Jun (Y170) polyclonal antibody detects endogenous levels of c-Jun protein only when phosphorylated at Tyr170.

BiowMW :~ 43 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :MAPK Erk in Growth and Differentiation Pathway,

Alternative Name :JUN; p39; V-jun avian sarcoma virus 17 oncogene homolog; Proto-oncogene c-Jun; AP1; Activator protein 1; Transcription factor AP-1

Immunogen :Synthetic phosphopeptide derived from human c-Jun around the phosphorylation site of Tyrosine 170.

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot (WB) analysis of p-c-Jun (Y170) pAb at 1:500 dilution Lane1:PC3 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:The Kidney tissue lysate of Rat(40ug) Lane4:The Kidney tissue lysate of Mouse(40ug)

Immunohistochemistry (IHC) analyzes of p-c-Jun (Y170) pAb in paraffin-embedded human lung carcinoma tissue at 1:50.showing nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Protection against UVB-Induced Photoaging by Nypa fruticans via Inhibition of MAPK/AP-1/MMP-1 Signaling

JournalOxidative Medicine and Cellular Longevity

ApplicationWB

IF5.076

SpeciesMouse

PMID32685089