Product Name :c-Jun (phospho-T93) polyclonal antibody
Reactivity :Human,Mouse,Rat
Application_all :WB: 1:500~1:1000
IHC: 1:50~1:200
IP: 1:50~1:200
Background :The c-Jun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus v-Jun oncogene. The c-Jun protein, along with c-Fos, is a component of the AP-1 transcriptional complex. c-Jun can form either Jun/Jun homodimers or Jun/Fos heterodimers via the leucine repeats in both proteins. Homo- and heterodimers bind to the TGACTCA consensus sequence present in numerous promoters and initially identified as the phorbol ester tumor promoter response element (TRE). Two additional genes, Jun B and Jun D, have been shown to be almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. All three form heterodimers among themselves and with c-Fos and other members of the Fos gene family.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :p-c-Jun (T93) polyclonal antibody detects endogenous levels of c-Jun protein only when phosphorylated at Thr93.
Note :For research use only, not for use in diagnostic procedure.
Pathway :MAPK Erk in Growth and Differentiation Pathway,
Alternative Name :JUN; p39; V-jun avian sarcoma virus 17 oncogene homolog; Proto-oncogene c-Jun; AP1; Activator protein 1; Transcription factor AP-1
Immunogen :Synthetic phosphopeptide derived from human c-Jun around the phosphorylation site of Threonine 93.
Modification :Phosphorylation
