产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

c-Jun (phospho-S73) polyclonal antibody（KU）

货号: BS4046

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS4046

Product Name :c-Jun (phospho-S73) polyclonal antibody（KU）

Swiss-Prot :P05412/P17535

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :The c-Jun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus v-Jun oncogene. The c-Jun protein, along with c-Fos, is a component of the AP-1 transcriptional complex. c-Jun can form either Jun/Jun homodimers or Jun/Fos heterodimers via the leucine repeats in both proteins. Homo- and heterodimers bind to the TGACTCA consensus sequence present in numerous promoters and initially identified as the phorbol ester tumor promoter response element (TRE). Two additional genes, Jun B and Jun D, have been shown to be almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. All three form heterodimers among themselves and with c-Fos and other members of the Fos gene family.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-c-Jun (S73) polyclonal antibody detects endogenous levels of c-Jun protein only when phosphorylated at Ser73.

BiowMW :~ 43 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :MAPK Erk in Growth and Differentiation Pathway,

Alternative Name :JUN; p39; V-jun avian sarcoma virus 17 oncogene homolog; Proto-oncogene c-Jun; AP1; Activator protein 1; Transcription factor AP-1

Immunogen :Synthetic phosphopeptide derived from human c-Jun around the phosphorylation site of Serine 73.

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot (WB) analysis of p-c-Jun (S73) pAb at 1:500 dilution Lane1:PC3 whole cell lysate(40ug) Lane2:U-87MG whole cell lysate(40ug) Lane3:PC12 whole cell lysate(40ug) Lane4:3T3-L1 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of p-c-Jun (S73) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

TRAF1 is a critical regulator of cerebral ischaemia–reperfusion injury and neuronal death

JournalNature Communications

ApplicationWB

IF11.878

SpeciesMouse,Human

PMID
ZNF16 (HZF1) promotes erythropoiesis and megakaryocytopoiesis via regulation of the c-KIT gene

JournalBiochemical Journal

ApplicationWB

IF4.097

SpeciesHuman

PMID24180487
Protective effect of celastrol in rat cerebral ischemia model: Down-regulating p-JNK, p-c-Jun and NF-κB

JournalBrain Research

ApplicationWB,IHC

IF2.733

SpeciesRat

PMID
Type II cGMP-dependent protein kinase inhibits ERK/JNK-mediated activation of transcription factors in gastric cancer cells

JournalMolecular Medicine Reports

ApplicationWB

IF2.1

SpeciesHuman

PMID
3,3'-Diindolylmethane attenuates cardiac H9c2 cell hypertrophy through 5'-adenosine monophosphate-activated protein kinase-α

JournalMolecular Medicine Reports

ApplicationWB

IF2.952

SpeciesRat

PMID25816057