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Cdc25C (phospho-S216) polyclonal antibody

货号: BS4035

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS4035

Product Name :Cdc25C (phospho-S216) polyclonal antibody

Swiss-Prot :P30307

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Cyclin dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits. The activity of cyclin dependent kinases is also regulated by the phosphorylation status, which is controlled by the antagonistic action of Wee1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1 S phase and G2 for CDC25B, CDC25C is predominantly expressed in G2.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-CDC25C (S216) polyclonal antibody detects endogenous levels of CDC25C protein only when phosphorylated at Ser216.

BiowMW :~ 60 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :G Protein-coupled Receptors Signaling to MAPK Erk,Cell Cycle G2 M DNA Damage Signaling Pathway,

Alternative Name :M-phase inducer phosphatase 3; Dual specificity phosphatase Cdc25C; CDC25C

Immunogen :Synthetic phosphopeptide derived from human Cdc25C around the phosphorylation site of Serine 216.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-CDC25C(S216) pAb at 1:500 dilution Lane1:PC12 whole cell lysate(40ug) Lane2:CT-26 whole cell lysate(40ug) Lane3:Hela whole cell lysate(40ug) Lane4:HEK293T whole cell lysate(40ug)