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Malic enzyme 1 (Y500) polyclonal antibody（KU）

货号: BS3886

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS3886

Product Name :Malic enzyme 1 (Y500) polyclonal antibody（KU）

Swiss-Prot :P48163

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :ME1 (Malic enzyme 1), also known as NADP-ME, MES or HUMNDME, is a 572 amino acid cytoplasmic protein that belongs to the malic enzyme family. Expressed ubiquitously with highest expression in liver and white adipose tissue, ME1 functions as an NADP-dependent enzyme that catalyzes the conversion of S-malate and NADP to pyruvate, carbon dioxide and NADPH (a reducing agent that participates in fatty acid biosynthesis). Through its ability to catalyze the reversible oxidative decarboxylation of malate, ME1 links the citric acid and glycolytic cycles. ME1 exists as a homotetramer that uses divalent metal cations, such as magnesium or manganese, as cofactors. The expression of ME1 is regulated by both thyroid hormone levels and the amount of carbohydrates in the diet, indicating that ME1 may play an important role as a housekeeping protein within the cell.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Malic enzyme 1 (Y500) polyclonal antibody detects endogenous levels of Malic enzyme 1 protein.

BiowMW :~ 64 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :malic enzyme 1; NADP(+)-dependent; cytosolic; HUMNDME; MES

Immunogen :Synthetic peptide, corresponding to amino acids 450-550 of Human Malic enzyme 1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Malic enzyme 1 (Y500) pAb at 1:2000 dilution Lane1:H1792 whole cell lysate(10ug) Lane2:HepG2 whole cell lysate(10ug) Lane3:The Testis tissue lysate of Mouse(10ug) Lane4:The Testis tissue lysate of Rat(10ug)