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ATP5I (K69) polyclonal antibody（KU）

货号: BS3813

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS3813

Product Name :ATP5I (K69) polyclonal antibody（KU）

Swiss-Prot :P56385

Host :Rabbit

Reactivity :Human,Mouse

Applications :WB IHC IP

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IP 1:50 - 1:100

Background :Mitochondrial ATP synthases (ATPases) transduce the energy contained in membrane electrochemical proton gradients into the energy required for synthesis of high-energy phosphate bonds. ATPases contain two linked complexes: F1, the hydrophilic catalytic core; and F0, the membrane-embedded protein channel. The two complexes are linked by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled, via a rotary mechanism of the central stalk subunits, with proton translocation across the membrane. ATP5I, also known as mitochondrial ATP synthase subunit E or ATP5K, is a 69 amino acid protein member of the ATPase E subunit family. Localized to the inner membrane of the mitochondria, ATP5I is a part of the F0 complex.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :ATP5I (K69) polyclonal antibody detects endogenous levels of ATP5I protein.

BiowMW :~ 12 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ATP synthase subunit e, mitochondrial; ATPase subunit e; ATP5I; ATP5K

Immunogen :Synthetic peptide, corresponding to amino acids 50-100 of Human ATP5I.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of ATP5I (K69) pAb at 1:500 dilution Lane1:AML-12 whole cell lysate(40ug) Lane2:C6 whole cell lysate(40ug) Lane3:HepG2 whole cell lysate(40ug) Lane4:PC3 whole cell lysate(40ug) Lane5:HEK293T whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of ATP5I (K69) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Immunoprecipitation of HEK293T cell lysate using ATP5I (K69) pAb (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) #BD0048 (lane 4 ) .Lane 1 is 30% input. The western blot was probed using ATP5I (K69) #BS3813. “↑”（supernatant）; “↓(deposition）