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Tak1 (D181) polyclonal antibody

货号: BS3745

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS3745

Product Name :Tak1 (D181) polyclonal antibody

Swiss-Prot :O43318

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a 74 kDa protein with intrinsic kinase activity towards serine/threonine residues and that is widely expressed in many tissue types and cell lines. Raf-1 activation is dependent on the small molecular weight GTPase Ras, but the means by which this activation occurs is poorly understood. Two proteins putatively involved in this process are Ksr-1 and Tak1. Ksr-1 (kinase suppressor of Ras) is a novel Raf-related protein kinase whose function is required for Ras signal transduction. Whether Ksr-1 lies directly downstream of Ras or acts in a parallel pathway is not yet known. Tak1 (TGFβ-activated kinase) has been shown to participate in the activation of the MAP kinase family in response to TGFß stimulation.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Tak1 (D181) polyclonal antibody detects endogenous levels of Tak1 protein.

BiowMW :~70, 82 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :SAPK JNK Signaling Cascades,Toll-like Receptor signaling,G Protein-coupled Receptors Signaling to MAPK Erk,MAPK Erk in Growth and Differentiation Pathway,

Alternative Name :Mitogen-activated protein kinase kinase kinase 7; Transforming growth factor-beta-activated kinase 1; TGF-beta-activated kinase 1; MAP3K7; TAK1

Immunogen :Synthetic peptide, corresponding to amino acids 151-200 of Human Tak1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Tak1 (D181) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:PC3 whole cell lysate(40ug) Lane3:SK-OVCAR3 whole cell lysate(40ug) Lane4:HCT116 whole cell lysate(40ug) Lane5:A549 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of Tak1 (D181) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Inhibition of Rho-kinase ameliorates myocardial remodeling and fibrosis in pressure overload and myocardial infarction: Role of TGF-β1–TAK1

JournalToxicology Letters

ApplicationWB

IF4.372

SpeciesMouse

PMID22465603
Pyridostigmine Ameliorates Cardiac Remodeling Induced by Myocardial Infarction via Inhibition of the Transforming Growth Factor-β1/TGF-β1–Activated Kinase Pathway

JournalJournal Of Cardiovascular Pharmacology

ApplicationWB

IF3.105

SpeciesRat

PMID24805145
Ginseng root extract attenuates inflammation by inhibiting the MAPK/NF-κB signaling pathway and activating autophagy and p62-Nrf2-Keap1 signaling in vitro and in vivo

JournalJournal Of Ethnopharmacology

ApplicationWB

IF4.36

SpeciesMouse

PMID34648903