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Laminin-R (Q215) polyclonal antibody（KU）

货号: BS3689

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS3689

Product Name :Laminin-R (Q215) polyclonal antibody（KU）

Swiss-Prot :P08865

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :Laminin receptor (Laminin-R) has a heterodimeric structure similar to that of receptors for other extracellular matrix proteins such as Fibronectin and Vitronectin. Incorporation of Laminin-R into lysosomal membranes makes it possible for lysosomes to attach to surfaces coated with Laminin. This and other properties identify Laminin-R as a member of the integrin family of cell adhesion receptors. The Laminin-R precursor is a polypeptide whose expression is consistently upregulated in aggressive carcinoma. Laminin-R (also known as colon carcinoma laminin-binding protein) and is found at nine-fold higher levels in colon carcinoma than in adjacent normal colonic epithelium. Additionally, the level of the Laminin-R is higher in the lung cancer cell line than in the lung cell line.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Laminin-R (Q215) polyclonal antibody detects endogenous levels of Laminin-R protein.

BiowMW :~ 40 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :40S ribosomal protein SA; 37 kDa laminin receptor precursor; 37LRP; 37/67 kDa laminin receptor; LRP/LR; 67 kDa laminin receptor; 67LR; Colon carcinoma laminin-binding protein; Laminin receptor 1; LamR; Laminin-binding protein precursor p40; LBP/p40; Multidrug resistance-associated protein MGr1-Ag; NEM/1CHD4; LAMBR; LAMR1; RPSA

Immunogen :Synthetic peptide, corresponding to amino acids 181-230 of Human Laminin-R.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Laminin-R (Q215) polyclonal antibody at 1:500 dilution Lane1:CT26 whole cell lysate(40ug) Lane2:PC12 whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:HEK293T whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of Laminin-R (Q215) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

IF image of BS3689 stained A549 cells.The cells were 4% paraformaldehyde fixed (20 min) and then incubated in 10% normal goat serum for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody Laminin-R (Q215) #BS3689(1:100) at 10µg/ml overnight at +4°C. The secondary antibody (Green) was Goat Anti-Rabbit IgG (H+L) FITC #BS10950 used at a 1/1000 dilution for 1h. Hoechst33342 #BD5011 was used to stain the cell nuclei (blue).