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Pepsin C (F306) polyclonal antibody（KU）

货号: BS3685

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS3685

Product Name :Pepsin C (F306) polyclonal antibody（KU）

Swiss-Prot :P20142

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Pepsin is one of the main proteolytic enzymes secreted by the gastric mucosa. Pepsin consists of a single polypeptide chain and arises from its precursor, pepsinogen, by removal of a 41 amino acid segment from the N-terminus. Pepsinogen is synthesized in the stomach lining, and hydrochloric acid, also produced by the gastric mucosa, is necessary to convert the inactive enzyme and to maintain the optimum acidity (pH 1-3) for pepsin function. Pepsin is particularly effective in cleaving peptide bonds involving aromatic amino acids. Pepsin shows extremely broad specificity, and although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. The amino acid composition of Pepsin C differs from those of pepsinogen and pepsin especially in the content of basic amino acids, glutamic acid, aspartic acid, leucine and isoleucine.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Pepsin C (F306) polyclonal antibody detects endogenous levels of Pepsin C protein .

BiowMW :~ 42 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Pepsinogen C; PGC; Gastricsin

Immunogen :Synthetic peptide, corresponding to amino acids 280-330 of Human Pepsin C.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Pepsin C (F306) pAb at 1:500 dilution Lane1:HepG2 whole cell lysate(40ug) Lane2:LO2 whole cell lysate(40ug) Lane3:SGC7901 whole cell lysate(40ug) Lane4:PMVEC whole cell lysate(40ug) Lane5:AML-12 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of Pepsin C (F306) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.