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Cortactin (Y460) polyclonal antibody

货号: BS3642

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS3642

Product Name :Cortactin (Y460) polyclonal antibody

Swiss-Prot :Q14247

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:2000 IHC: 1:50~1:100 IF: 1:50~1:100

Background :Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino terminal tandem repeats of a unique 37 amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha helical structure followed by a proline rich region. Between the proline rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr421, Tyr470, and Tyr486). In vitro, cortactin binds to and cross links F actin into meshworks.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Cortactin (Y460) polyclonal antibody detects endogenous levels of Cortactin protein.

BiowMW :~ 85 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Actin Dynamics,Contribution of ECM and Cytoskeletal Factors to EMT,Adherens Junction signaling,

Alternative Name :Src substrate cortactin; Amplaxin; Oncogene EMS1; CTTN; EMS1

Immunogen :Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human Cortactin (NP_612632.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using Cortactin antibody at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded human lung cancer using Cortactin antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human mammary cancer using Cortactin antibody (A15054) at dilution of 1:100 (40x lens).