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Paxillin (L25) polyclonal antibody

货号: BS3585

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS3585

Product Name :Paxillin (L25) polyclonal antibody

Swiss-Prot :P49023

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :Paxillin is a multidomain protein that localizes primarily to focal adhesion sites in the extracellular matrix. Paxillin is one of the key components of integrin signaling, and tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization. Paxillin is phosphorylated by another focal adhesion component, focal adhesion kinase (FAK), at Tyr118. Phospho-Paxillin (Tyr118) may provide a docking site for recruitment of other signaling molecules to focal adhesions. It has been shown that the SH2 domain of Crk binds to the phosphorylated Tyr118 of paxillin.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Paxillin (L25) polyclonal antibody detects endogenous levels of Paxillin protein.

BiowMW :~ 54,68 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Actin Dynamics,Tumor Angiogenesis,Contribution of ECM and Cytoskeletal Factors to EMT,

Alternative Name :Paxillin; PXN

Immunogen :Synthetic peptide, corresponding to amino acids N-terminus of Human Paxillin.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of PXN polyclonal antibody at 1:1000 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:Hela whole cell lysate(40ug) Lane3:H460 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug) Lane5:CT-26 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of Paxillin (L25) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

lncRNA H19 regulates epithelial–mesenchymal transition and metastasis of bladder cancer by miR-29b-3p as competing endogenous RNA

JournalBiochimica Et Biophysica Acta-Molecular Cell Research

ApplicationWB

IF4.105

SpeciesHuman

PMID28779971