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NGF (L82) polyclonal antibody（KU）

货号: BS3564

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS3564

Product Name :NGF (L82) polyclonal antibody（KU）

Swiss-Prot :P01138

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :The prototype neurotrophin is nerve growth factor (NGF), originally discovered in the 1950s as a soluble peptide promoting the survival of, and neurite outgrowth from, sympathetic ganglia. Three additional structurally homologous neurotrophic factors have been identified. These include brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and neurotrophin-4 (NT-4) (also designated NT-5). These various neurotrophins stimulate the in vitro survival of distinct, but partially overlapping, populations of neurons. The cell surface receptors through which neurotrophins mediate their activity have been identified. For instance, the Trk A receptor is the preferential receptor for NGF, but also binds NT-3 and NT-4.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :NGF (L82) polyclonal antibody detects endogenous levels of Pro-NGF protein, this antibody does not cross-react with Mature-NGF.

BiowMW :~ 34 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Beta-nerve growth factor; Beta-NGF; NGFB

Immunogen :Synthetic peptide, corresponding to amino acids 51-100 of Human NGF.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of NGF (L82) pAb at 1:500 dilution Lane1:HCT116 whole cell lysate(40ug) Lane2:Panc1 whole cell lysate(40ug) Lane3:CT26 whole cell lysate(40ug) Lane4:H9C2 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of NGF (L82) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Effect of nerve growth factor on the proliferation in newborn bovine testicular Sertoli cells

JournalReproduction

ApplicationIF,WB

IF3.206

SpeciesBovine

PMID32567558