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PDGF-A (K154) polyclonal antibody（KU）

货号: BS3517

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS3517

Product Name :PDGF-A (K154) polyclonal antibody（KU）

Swiss-Prot :P04085

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :PDGF is a mitogen for mesenchyme- and glia-derived cells. It consists of two disulfide-bonded polypeptide chains, A and B, and occurs as three isoforms, PDGF-AA, PDGF-AB and PDGF-BB. The three isoforms bind with different affinities to two receptor types, α and β, which are structurally related and endowed with protein-tyrosine kinase domains. Ligand binding induces activation of the receptor kinases by formation of receptor dimers; the A subunit of PDGF binds only to α receptors with high affinity, whereas the B subunit can bind to both α and β receptors. Evidence suggests that PDGF may function as a neurotrophic factor. The fact that receptors for PDGF-A are expressed in oligodendrocyte progenitor cells whereas receptors for PDGF-B are expressed on neurons suggests that the different isoforms of PDGF may regulate growth and differentiation of different cell types in the developing central nervous system by paracrine and autocrine routes.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PDGF-A (K154) polyclonal antibody detects endogenous levels of PDGF-A protein.

BiowMW :~ 24 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Platelet-derived growth factor subunit A; PDGF subunit A; PDGF-1; Platelet-derived growth factor A chain; Platelet-derived growth factor alpha polypeptide; PDGFA; PDGF1

Immunogen :Synthetic peptide, corresponding to amino acids 121-160 of Human PDGF-A.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of PDGF-A (K154) pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:Hela whole cell lysate(40ug) Lane3:The Lung tissue lysate of Mouse(40ug) Lane4:PMVEC whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of PDGF-A (K154) pAb in paraffin-embedded human lung carcinoma tissue at 1:100.

Sublytic C5b-9 triggers glomerular mesangial cell proliferation via enhancing FGF1 and PDGFα gene transcription mediated by GCN5-dependent SOX9 acetylation in rat Thy-1 nephritis

JournalFaseb Journal

ApplicationWB,IHC

IF5.191

SpeciesRat,Human

PMID34156114
ERK1/2-dependent activity of SOX9 is required for sublytic C5b-9-induced expression of FGF1, PDGFα, and TGF-β1 in rat Thy-1 nephritis

JournalINTERNATIONAL IMMUNOPHARMACOLOGY

ApplicationWB

IF5.6

SpeciesRat

PMID38118314