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Nephrin (H892) polyclonal antibody

货号: BS3462

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS3462

Product Name :Nephrin (H892) polyclonal antibody

Swiss-Prot :O60500

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:100~1:500

Background :Nephrin is a member of the immunoglobulin family of cell adhesion molecules that localizes to opposing sites of the secondary foot processes formed by podocytes, a specialized epithelial cell that ensures size- and charge-selective ultrafiltration. The human nephrin gene maps to chromosome 19q13.1 and encodes a 1,241 amino acid protein that contains a transmembrane domain, 8 Ig-like modules, and 1 fibronectin III-like module. Nephrin is expressed in embryonic and adult kidneys and localizes to glomerular podocytes and the glomerular slit diaphragm. Nephrin stimulates mitogen-activated protein kinases and is enhanced by podocin, which binds to the cytoplasmic tail of nephrin.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Nephrin (H892) polyclonal antibody detects endogenous levels of Nephrin protein.

BiowMW :~ 135 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Nephrin; Renal glomerulus-specific cell adhesion receptor; NPHS1; NPHN

Immunogen :Synthetic peptide, corresponding to amino acids 861-910 of Human Nephrin.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of Nephrin (H892) polyclonal antibody at 1:1000 dilution Lane1:The muscle tissue lysate of Rat(47ug) Lane2:HEK293T whole cell lysate(41ug) Lane3:Jurkat whole cell lysate(41ug) Lane4:H9C2 whole cell lysate(60ug)

Immunohistochemistry (IHC) analyzes of Nephrin (H892) pAb in paraffin-embedded human colorectal cancer carcinoma tissue at 1:100.

Immunofluorescence analysis of BV2 cells using Nephrin (H892) pAb at dilution of 1:200 (40x lens).

C-peptide ameliorates high glucose-induced podocyte dysfunction through the regulation of the Notch and TGF-β signaling pathways

JournalPeptides

ApplicationWB

IF3.75

SpeciesMouse

PMID33901627