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N4BP1 (Y415) polyclonal antibody（KU）

货号: BS3340

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS3340

Product Name :N4BP1 (Y415) polyclonal antibody（KU）

Swiss-Prot :O75113

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Nedd4-binding partner-1 (N4BP1) has been identified as a protein interactor and a substrate of the homologous to E6AP C terminus (HECT) domain-containing E3 ubiquitin–protein ligase (E3), Nedd4. Here, we describe a previously unrecognized functional interaction between N4BP1 and Itch, a Nedd4 structurally related E3, which contains four WW domains, conferring substrate-binding activity. We show that N4BP1 association with the second WW domain (WW2) of Itch interferes with E3 binding to its substrates. In particular, we found that N4BP1 and p73α, a target of Itch-mediated ubiquitin/proteasome proteolysis, share the same binding site. By competing with p73α for binding to the WW2 domain, N4BP1 reduces the ability of Itch to recruit and ubiquitylate p73α and inhibits Itch autoubiquitylation activity both in in vitro and in vivo ubiquitylation assays.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :N4BP1 (Y415) polyclonal antibody detects endogenous levels of N4BP1 protein.

BiowMW :~ 120 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :NEDD4 binding protein 1; N4BP1; KIAA0615

Immunogen :Synthetic peptide, corresponding to amino acids 391-440 of Human N4BP1.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of N4BP1 (Y415) pAb at 1:1000 dilution Lane1:A2780 whole cell lysate(20ug) Lane2:A549 whole cell lysate(20ug) Lane3:HEK293 whole cell lysate(20ug) Lane4:PC12 whole cell lysate(40ug) Lane5:AML-12 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of N4BP1 (Y415) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.