产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

Vimentin (S55) polyclonal antibody

货号: BS3182

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS3182

Product Name :Vimentin (S55) polyclonal antibody

Swiss-Prot :P08670

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. Intermediate filaments are constructed from two-chain, α-helical, coiled-coil molecules arranged on an imperfect helical lattice and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. One such intermediate filament protein, Vimentin, is a general marker of cells originating in the mesenchyme. Vimentin is frequently coexpressed with other members of the intermediate filament family, such as the cytokeratins, in neoplasms including melanoma and breast carcinoma.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Vimentin (S55) polyclonal antibody detects endogenous levels of Vimentin protein.

BiowMW :~ 40, 57 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Neuronal and Glial Cell Marker Atlas,Contribution of ECM and Cytoskeletal Factors to EMT,

Alternative Name :Vimentin; VIM

Immunogen :Synthetic peptide, corresponding to amino acids 111-60 of Human Vimentin.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Vimentin (S55) polyclonal antibody at 1:500 dilution Lane1:RAW264.7 whole cell lysate(40ug) Lane2:PC12 whole cell lysate(40ug) Lane3:NIH-3T3 whole cell lysate(40ug) Lane4:Jurkat whole cell lysate(40ug)