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POLR2E (L58) polyclonal antibody

货号: BS3113

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS3113

Product Name :POLR2E (L58) polyclonal antibody

Swiss-Prot :P19388

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :POLR2E (L58) polyclonal antibody detects endogenous levels of POLR2E protein.

BiowMW :~ 25 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :DNA-directed RNA polymerases I, II, and III subunit RPABC1; RNA polymerases I, II, and III subunit ABC1; DNA-directed RNA polymerase II 23 kDa polypeptide; DNA-directed RNA polymerase II subunit E; RPB5 homolog; XAP4; POLR2E

Immunogen :Synthetic peptide, corresponding to amino acids 30-80 of Human POLR2E.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of POLR2E (L58) polyclonal antibody at 1:500 dilution Lane1:CT26 whole cell lysate(40ug) Lane2:PC12 whole cell lysate(40ug) Lane3:PC3 whole cell lysate(40ug) Lane4:HCT116 whole cell lysate(40ug) Lane5:786-O whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of POLR2E (L58) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.