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PUMA (R169) polyclonal antibody（KU）

货号: BS2922

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS2922

Product Name :PUMA (R169) polyclonal antibody（KU）

Swiss-Prot :Q9BXH1

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :PUMA (Bcl-2 binding component 3, JFY1, PUMA/JFY1) is a mitochondrial pro-apoptotic Bcl-2 homology domain (BH3)-only protein that induces rapid apoptosis through a Bax- and mitochondria-dependent pathway. The PUMA gene encodes four proteins originating from different splice variants of the same transcript: PUMAα, β, γ and δ. Both PUMAα and PUMAβ contain a BH3 domain, while PUMAγ and PUMAδ lack this domain. The BH3 domain is essential for binding of PUMAα and PUMAβ to Bcl-2 or Bcl-xL. PUMA is an initiator of γ-radiation apoptosis and glucocorticoid-induced apoptosis in lymphoid cells in vivo. Bcl-2 family members generally regulate apoptosis and transmit death signals to mitochondria. Members of this family include both pro- and anti-apoptotic proteins that share homologous sequences known as Bcl-2 homology domains (BH1-4). The BH3 proteins, BID, NOXA, PUMA, NBK, Bim and Bad, are all pro-apoptotic and share sequence homology within the amphipathic α-helical BH3 region.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PUMA (R169) polyclonal antibody detects endogenous levels of PUMA protein.

BiowMW :~ 15, 20, 28 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Apoptosis,Mitochondrial Control of Apoptosis,

Alternative Name :Bcl-2-binding component 3; JFY-1; p53 up-regulated modulator of apoptosis; BBC3; PUMA

Immunogen :Synthetic peptide, corresponding to amino acids 132-183 of Human PUMA.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of PUMA (R169) polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate(40ug) Lane2:HepG2 whole cell lysate(40ug) Lane3:MCF-7 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug) Lane5:BV2 whole cell lysate(40ug)

MEHP-induced oxidative DNA damage and apoptosis in HepG2 cells correlates with p53-mediated mitochondria-dependent signaling pathway

JournalFood And Chemical Toxicology

ApplicationWB

IF4.679

SpeciesHuman

PMID22542555
Mono(2‐ethylhexyl) phthalate induces apoptosis in p53‐silenced L02 cells via activation of both mitochondrial and death receptor pathways

JournalEnvironmental Toxicology

ApplicationWB

IF3.118

SpeciesHuman

PMID
Depletion of NFBD1/MDC1 Induces Apoptosis in Nasopharyngeal Carcinoma Cells Through the p53–ROS–Mitochondrial Pathway

JournalOncology Research

ApplicationWB

IF5.574

SpeciesHuman

PMID28081741
Silence of long noncoding RNA PANDAR switches low-dose curcumin-induced senescence to apoptosis in colorectal cancer cells

JournalOncoTargets and Therapy

ApplicationWB

IF4.147

SpeciesHuman

PMID28176943
NFBD1/MDC1 participates in the regulation of proliferation and apoptosis in human laryngeal squamous cell carcinoma

JournalClinical & Translational Oncology

ApplicationWB

IF2.737

SpeciesHuman

PMID28921460