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C/EBP-γ (E64) polyclonal antibody

货号: BS2558

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS2558

Product Name :C/EBP-γ (E64) polyclonal antibody

Swiss-Prot :P53567

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :The transcription factor C/EBP α (CCAAT-enhancer binding protein) is a heatstable, sequence-specific DNA-binding protein first purified from rat liver nuclei that binds avidly to several different cis-regulatory DNA sequences commonly associated with viral and cellular genes transcribed by RNA polymerase II. C/EBP α regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP α uses a bipartite structural motif to bind DNA. Two protein chains dimerize through a set of amphipathic alpha helices termed the leucine zipper. Highly basic polypeptide regions emerge from the zipper to form a linked set of DNA contact surfaces. C/EBP α appears to function exclusively in terminally differentiated, growth-arrested cells. Additional family members include C/EBP β, C/EBP γ,C/EBP δ and C/EBP ε, all of which exhibit similar DNA-binding specificities and affinities to C/EBP α. Furthermore, C/EBP β and C/EBP δ readily form heterodimers both with each other as well as with C/EBP α.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :C/EBP-γ (E64) detects endogenous levels of C/EBP-γ protein.

BiowMW :~ 22 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CCAAT/enhancer-binding protein gamma; C/EBP gamma; CEBPG; C/EBPγ; CEBPγ

Immunogen :Synthetic peptide, corresponding to amino acids 50-100 of Human C/EBP-γ.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of C/EBP-γ (E64) polyclonal antibody at 1:500 dilution Lane1:SGC-7901 whole cell lysate(40ug) Lane2:The testis tissue lysate of Mouse(30ug)

Immunohistochemistry (IHC) analyzes of C/EBP-γ (E64) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.