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Smad1/5/9 (Q22) polyclonal antibody

货号: BS2486

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS2486

Product Name :Smad1/5/9 (Q22) polyclonal antibody

Swiss-Prot :Q15797/Q99717/O15198

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :IHC

Application_all :IHC: 1:50~1:200

Background :Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Smad1/5/9 (Q22) polyclonal antibody detects endogenous levels of Smad1/5/9 protein.

BiowMW :~ 60 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Contribution of Soluble Factors to EMT,hippo Signaling,

Alternative Name :Mothers against decapentaplegic homolog 1; MAD homolog 1; Mothers against DPP homolog 1; JV4-1; Mad-related protein 1; SMAD family member 1; SMAD 1; Smad1; hSMAD1; Transforming growth factor-beta-signaling protein 1; BSP-1; SMAD1; BSP1; MADH1; MADR1; Mothers against decapentaplegic homolog 5; MAD homolog 5; Mothers against DPP homolog 5; V5-1; SMAD family member 5; SMAD 5; Smad5; hSmad5; SMAD5; MADH5; Mothers against decapentaplegic homolog 9; MAD homolog 9; Mothers against DPP homolog 9; Madh6; SMAD family member 9; SMAD 9; Smad9; SMAD9; MADH6; MADH9

Immunogen :Synthetic peptide, corresponding to the N-terminal of Human Smad1.

Conjugate :Unconjugated

Modification :Unmodification

Immunohistochemistry (IHC) analyzes of Smad1/5/9 (Q22) pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of Smad1/5/9 (Q22) pAb in paraffin-embedded human colon carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining.Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of Smad1/5/9 (Q22) pAb in paraffin-embedded human liver carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining.Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.