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VAV2 (D138) polyclonal antibody（KU）

货号: BS2443

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS2443

Product Name :VAV2 (D138) polyclonal antibody（KU）

Swiss-Prot :P52735

Host :Rabbit

Reactivity :Human,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :VAV2 is a ubiquitously expressed structural homolog of the VAV protooncogene that is expressed preferentially in hematopoetic cells. Both proteins are comprised of a Dbl homology (DH) domain with guanosine nucleotide exchange (GEF) activity exclusively directed towards Rho/Rac GTPases, a pleckstrin homology (PH) domain, a calponin-homology (CH) region, an acidic domain (AD) a zinc finger butterfly motif, two SH3 regions and one SH2 domain. GEF activity of RhoA family G proteins is induced by tyrosine phosphorylation in wild type VAV2, and is constitutively activated in N terminus deleted oncogene forms. Constitutive expression of a VAV2 oncoprotein may result in morphological alterations including highly enlarged cells in which karyokinesis and cytokinesis frequently are uncoupled.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :VAV2 (D138) polyclonal antibody detects endogenous levels of VAV2 protein.

BiowMW :~108 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Actin Dynamics,

Alternative Name :Guanine nucleotide exchange factor VAV2; VAV-2; VAV2

Immunogen :Synthetic peptide, corresponding to amino acids 100-150 of Human VAV2.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of VAV2 (D138) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate(20ug) Lane2:Hela whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:C6 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of VAV2 (D138) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing cytoplasmic staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.