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NF-YC (W85) polyclonal antibody

货号: BS2428

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS2428

Product Name :NF-YC (W85) polyclonal antibody

Swiss-Prot :Q13952

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :The CCAAT-binding factor NF-Y is a heteromeric transcription factor that specifically binds to CCAAT sequences in many eukaryotic genes. NF-Y is made up of three subunits, NF-YA, NF-YB and NF-YC. All three components are necessary for DNA binding. In each NF-Y subunit, the segment needed for formation of the NF-Y-DNA complex is conserved from yeast to human. These conserved segments are homologous to the histone-fold motif of eukaryotic histones. The DNA binding domains of the NF-YB and NF-YC subunits have been suggested to interact through a protein-protein histone-fold “handshake” motif in a manner analogous to the histone proteins, H2B and H2A, respectively.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :NF-YC (W85) polyclonal antibody detects endogenous levels of NF-YC protein.

BiowMW :~ 50 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Nuclear transcription factor Y subunit gamma; CAAT box DNA-binding protein subunit C; Nuclear transcription factor Y subunit C; NF-YC; Transactivator HSM-1/2; NFYC

Immunogen :Synthetic peptide, corresponding to amino acids 50-106 of Human NF-YC.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of NF-YC (W85) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate

Immunohistochemistry (IHC) analyzes of NF-YC (W85) pAb in paraffin-embedded liver cancer tissue at 1:100.