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Rad51C (E185) polyclonal antibody

货号: BS2421

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS2421

Product Name :Rad51C (E185) polyclonal antibody

Swiss-Prot :O43502

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :The process of homologous recombination is a major DNA repair pathway that operates on DNA double-strand breaks to promote error-free repair. Human Rad51 protein assembles on a DNA molecule through cooperative binding and forms a long filament for homologous recombination. In addition to Rad51 protein, five paralogs have been identified in vertebrates, Rad51B, Rad51C, Rad51D, Xrcc2 and Xrcc3. Rad51C binds single- and double-stranded DNA and preferentially binds 3'-end-tailed double-stranded DNA. Rad51C is expressed in a variety of tissues, with highest expression in testis, heart muscle, spleen and prostate.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Rad51C (E185) polyclonal antibody detects endogenous levels of Rad51C protein.

BiowMW :~ 50 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :DNA repair protein RAD51 homolog 3; R51H3; RAD51 homolog C; RAD51-like protein 2; RAD51C; RAD51L2

Immunogen :Synthetic peptide, corresponding to amino acids 161-210 of Human Rad51C.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Rad51C (E185) pAb at 1:500 dilution Lane1:H9C2 whole cell lysate(40ug) Lane2:The Testis tissue lysate of Mouse(40ug) Lane3:MCF-7 whole cell lysate(40ug) Lane4:Hela whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of Rad51C (E185) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.