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Tsg 101 (E303) polyclonal antibody

货号: BS2347

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS2347

Product Name :Tsg 101 (E303) polyclonal antibody

Swiss-Prot :Q99816

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :It is now well accepted that the transformation of a normal cell to one that is malignant can result from mutations in genes that encode proteins with key regulatory functions. Examples include the retinoblastoma gene product (Rb p110), p53, VHL and APC. Using a novel cloning strategy that allows the isolation of previously uncharacterized genes encoding selectable recessive phenotypes, an additional tumor suppressor gene has been identified. This gene, termed tsg 101 for tumor susceptibility gene 101, encodes a stathmin binding domain protein. When expression of this growth inhibitory gene is blocked in NIH/3T3 cells using antisense mRNA, the cells exhibit a transformed phenotype and are tumorigenic in SL6 mice.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Tsg 101 (E303) polyclonal antibody detects endogenous levels of Tsg 101 protein.

BiowMW :~45 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Tumor susceptibility gene 101 protein; ESCRT-I complex subunit TSG101; TSG101

Immunogen :Synthetic peptide, corresponding to amino acids 268-320 of Human Tsg 101.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Tsg 101 (E303) polyclonal antibody in extracts from HEK293A cells.

Immunohistochemistry (IHC) analyzes of Tsg 101 (E303) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing nucleus/cell membrane and cytoplasmic staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of Tsg 101 (E303) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Exosomes with low miR-34c-3p expression promote invasion and migration of non-small cell lung cancer by upregulating integrin α2β1

JournalSignal Transduction and Targeted Therapy

ApplicationWB

IF13.493

SpeciesHuman

PMID32317629