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MELK (N449) polyclonal antibody（KU）

货号: BS2309

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS2309

Product Name :MELK (N449) polyclonal antibody（KU）

Swiss-Prot :Q14680

Host :Rabbit

Reactivity :Human,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :Maternal embryonic leucine zipper kinase (KIAA0175, HPK38) or MELK, a new member of the Snf1/AMPK family of kinases, encodes a protein with a kinase catalytic domain and a leucine zipper motif consisting of a periodic repetition of leucine residues at every seventh residue located within the N-terminal catalytic domain. This motif has been observed in myriad DNA-binding proteins and is presumed to be involved in protein-DNA interactions, and potentially protein-protein interactions. Research predicts that the gene product of MELK plays a role in the signal transduction events in the egg and early embryo. Mouse and human MELK proteins share 95% sequence identity in the kinase domain and northern blot analysis in mouse indicates that MELK expression is restricted to spermatogonia in the testis and to oocytes in the ovary.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :MELK (N449) polyclonal antibody detects endogenous levels of MELK protein.

BiowMW :~ 75 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Maternal embryonic leucine zipper kinase; hMELK; Protein kinase Eg3; pEg3 kinase; Protein kinase PK38; hPK38; Tyrosine-protein kinase MELK; MELK; KIAA0175; Pk38; mPK38

Immunogen :Synthetic peptide, corresponding to amino acids 415-473 of Human MELK.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of MELK (N449) pAb at 1:500 dilution Lane1:C6 whole cell lysate(40ug) Lane2:SK-OVCAR3 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(20ug) Lane4:Hela whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of MELK (N449) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.