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MEK-4 (K350) polyclonal antibody（KU）

货号: BS2276

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS2276

Product Name :MEK-4 (K350) polyclonal antibody（KU）

Swiss-Prot :P45985

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF IP

Application_all :WB: 1:500~1:1000 IHC/IF: 1:50~1:200 IP: 1:10~1:100

Background :The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK 5, whereas MEK-6 phosphorylates p38 and p38β. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Western blot (WB) analysis of MEK-4 (K350) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:AML-12 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug) Lane5:HEK293T whole cell lysate(40ug)

BiowMW :~ 44 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :SAPK JNK Signaling Cascades,Signaling Pathways Activating p38 MAP KinaseMAPK Erk in Growth and Differentiation Pathway,

Alternative Name :Dual specificity mitogen-activated protein kinase kinase 4; MAP kinase kinase 4; MAPKK 4; JNK-activating kinase 1; MAPK/ERK kinase 4; MEK 4; SAPK/ERK kinase 1; SEK1; Stress-activated protein kinase kinase 1; SAPK kinase 1; SAPKK-1; SAPKK1; c-Jun N-terminal kinase kinase 1; JNKK; MAP2K4; JNKK1; MEK4; MKK4;; PRKMK4; SEK1; SERK1; SKK1

Immunogen :Synthetic peptide, corresponding to amino acids 330-380 of Human MEK-4.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of MEK-4 (K350) pAb at 1:1000 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:The Brain tissue lysate of Mouse(20ug) Lane4:The Brain tissue lysate of Rat(40ug)

Immunohistochemistry (IHC) analyzes of MEK-4/ MAP2K4 (K350) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cell membrane cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of MEK-4/ MAP2K4 (K350) pAb in paraffin-embedded human lung carcinoma tissue at 1:50.showing cell membrane cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.