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TBP (A184) polyclonal antibody

货号: BS2262

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS2262

Product Name :TBP (A184) polyclonal antibody

Swiss-Prot :P20226

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :IHC

Application_all :IHC: 1:50~1:200

Background :The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This protein is not suitable for samples where the nuclear envelope has been removed.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :TBP (A184) polyclonal antibody detects endogenous levels of TBP protein..

BiowMW :~ 45 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :TATA-box-binding protein; TATA sequence-binding protein; TATA-binding factor; TATA-box factor; Transcription initiation factor TFIID TBP subunit; TBP; GTF2D1; TF2D; TFIID

Immunogen :Synthetic peptide, corresponding to amino acids 150-200 of Human TBP.

Conjugate :Unconjugated

Modification :Unmodification

Immunohistochemistry (IHC) analyzes of TBP (A184) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.