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TANK (F194) polyclonal antibody

货号: BS2231

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS2231

Product Name :TANK (F194) polyclonal antibody

Swiss-Prot :Q92844

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :The tumor necrosis factor (TNF) receptor superfamily is composed of several type I integral membrane glycoproteins that exhibit homology in their cystine- rich extracellular domains. Members of this family include TNF-RI and -RII, FAS, OX40, CD27, CD30 and CD40. Ligands for these receptors can be small, secreted proteins such as TNF, or type II integral membrane proteins, such as the CD40 ligand, CD40L. While the signal transduction mechanism of the TNF receptor superfamily is poorly understood, stimulation of cells with either TNF or soluble CD40L has been shown to induce the nuclear translocation of NFκB. Members of the TRAF family associate with activated TNF-R and CD40 and have been implicated in this process. The discovery of a novel protein, designated TANK, has shed light on the means by which TRAF activation of NFkB occurs. TANK is not only capable of binding to all three TRAFs, but also of synergizing with TRAF2 to activate the NFκB signaling cascade. TANK contains a regulatory carboxy terminal domain that maintains its inactivity in unstimulated cells. Upon its association with TRAF2, the inhibitory effect of this domain is overcome.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :TANK (F194) polyclonal antibody detects endogenous levels of TANK protein.

BiowMW :~ 50 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :NF-kB Signaling,STING Signaling,

Alternative Name :TRAF family member-associated NF-kappa-B activator; TRAF-interacting protein; I-TRAF; TANK; ITRAF; TRAF2

Immunogen :Synthetic peptide, corresponding to amino acids 160-203 of Human TANK.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of TANK pAb at 1:500 dilution Lane1:HepG2 whole cell lysate(40ug) Lane2:RAW264.7 whole cell lysate(40ug) Lane3:PC12 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of TANK (F194) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50.showing cytoplasmic staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

MAVS activates TBK1 and IKKε through TRAFs in NEMO dependent and independent manner

JournalPlos Pathogens

ApplicationIP

IF6.463

SpeciesHuman

PMID29125880