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SMG7 (K548) polyclonal antibody（KU）

货号: BS2112

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS2112

Product Name :SMG7 (K548) polyclonal antibody（KU）

Swiss-Prot :Q92540

Host :Rabbit

Reactivity :Human

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :In humans, NMD depends on RNA-dependent ATPase and 5' to 3' helicase UPF1, plus six other proteins designated SMG1, SMG5, SMG6, SMG7, UPF2 and UPF3. SMG5, SMG7 and UPF1 localize to cytoplasmic foci called P-bodies, while SMG5, SMG6 and SMG7 target UPF1 for dephosphorylation. SMG7 may also act as an adaptor in targeting mRNAs associated with phosphorylated UPF1 for degradation. SMG7 provides a link between the NMD pathway and mRNA degradation machinery by forming a complex with the proteins SMG5 and UPF1, interacting with them via its N-terminal domain, and targeting bound reporter transcripts for decay via its C-terminal domain. SMG7 contains a 14-3-3-like domain, and residues that bind phosphoserine-containing peptides in 14-3-3 proteins are conserved at the equivalent positions in SMG7.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :SMG7 (K548) polyclonal antibody detects endogenous levels of SMG7 protein.

BiowMW :~ 127 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :C1orf16; EST1 like protein C; EST1 telomerase component homolog C; EST1C; ever shorter telomeres 1C; FLJ23717; SGA56M; SMG 7;

Immunogen :Synthetic peptide, corresponding to amino acids 512-564 of Human SMG7.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of SMG7 (K548) pAb at 1:500 dilution Lane1:K562 whole cell lysate(40ug) Lane2:MCF-7 whole cell lysate(40ug) Lane3:U-87MG whole cell lysate(40ug) Lane4:SGC7901 whole cell lysate(40ug) Lane5:PC12 whole cell lysate(40ug) Lane6:BV2 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of SMG7 (K548) pAb in paraffin-embedded human tonsil cancer tissue at 1:50.