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Smad4 (L43) polyclonal antibody（KU）

货号: BS2050

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS2050

Product Name :Smad4 (L43) polyclonal antibody（KU）

Swiss-Prot :Q13485

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IF

Application_all :WB: 1:10000~1:20000 IF: 1:100~1:500

Background :Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Smad4 (L43) polyclonal antibody detects endogenous levels of Smad4 protein.

BiowMW :~ 75 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Cell Cycle Control G1 S Checkpoint,Contribution of Soluble Factors to EMT,hippo Signaling,

Alternative Name :Mothers against decapentaplegic homolog 4; MAD homolog 4; Mothers against DPP homolog 4; Deletion target in pancreatic carcinoma 4; SMAD family member 4; SMAD 4; Smad4; hSMAD4; SMAD4; DPC4; MADH4

Immunogen :Synthetic peptide, corresponding to Human Smad4.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of Smad4 (L43) polyclonal antibody at 1:10000 dilution Lane1:Hela whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:BV2 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug) Lane5:THP-1 whole cell lysate(40ug) Lane6:HepG2 whole cell lysate(40ug)

Immunofluorescence analysis of Hela cells using CD326 pAb at dilution of 1:200 (40x lens).

Well-differentiated pancreatic ductal adenocarcinomas measuring ≤1 cm exhibit early features of tumor progression: a report of five lesions and a comparative study with advanced lesions

JournalSurgery Today

ApplicationIHC

IF2.549

SpeciesHuman

PMID
Functional screen reveals essential roles of miR‐27a/24 in differentiation of embryonic stem cells

JournalEmbo Journal

ApplicationWB

IF11.227

SpeciesMouse

PMID25519956
miR-224 Controls Human Colorectal Cancer Cell Line HCT116 Proliferation by Targeting Smad4

JournalInternational Journal of Medical Sciences

ApplicationWB

IF2.333

SpeciesHuman

PMID28924364
Dental and periodontal phenotypes of Dlx2 overexpression in mice

JournalMolecular Medicine Reports

ApplicationIHC

IF1.851

SpeciesMouse

PMID28447749
Gambogic acid suppresses cancer invasion and migration by inhibiting TGFβ1-induced epithelial-to-mesenchymal transition

JournalOncotarget

ApplicationWB

IF0

SpeciesHuman

PMID28404892