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CBP20 (R21) polyclonal antibody（KU）

货号: BS2012

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS2012

Product Name :CBP20 (R21) polyclonal antibody（KU）

Swiss-Prot :P52298

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF IP

Application_all :WB: 1:500~1:1000 IHC/IF: 1:50~1:200 IP: 1:10~1:100

Background :In eukaryotes, the majority of mRNAs have an m(7)G cap, which is added cotranscriptionally and plays a critical role in many aspects of mRNA metabolism. The effect of the cap on translation is mediated by the initiation factor eIF-4F, whereas the effect on pre-mRNA splicing involves a nuclear complex (CBC). CBC consists of two cap binding proteins, CBP20 and CBP80, which mediate the stimulatory functions of the cap in pre-mRNA splicing, 3' end formation and U snRNA export. The genes CBC1 and CBC2 encode CBP80 and CBP20, respectively. CBP80 comprises three domains, each containing a MIF4G domain. CBP20 has an RNAP fold and associates with the second and third domains of CBP80.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :CBP20 (R21) polyclonal antibody detects endogenous levels of CBP20 protein.

BiowMW :~ 18 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Nuclear cap-binding protein subunit 2; 20 kDa nuclear cap-binding protein; Cell proliferation-inducing gene 55 protein; NCBP 20 kDa subunit; CBP20; NCBP-interacting protein 1; NIP1; NCBP2; CBP20; ORF Names: PIG55

Immunogen :Synthetic peptide, corresponding to amino acids N-terminus of Human CBP20.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of CBP20 (R21) pAb at 1:1000 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:L02 whole cell lysate(40ug) Lane3:CT26 whole cell lysate(40ug) Lane4:H9C2 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of CBP20 (R21) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunoprecipitation of HEK293T cell lysate using CBP20 (R21) pAb (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) #BD0048 (lane 4 ) .Lane 1 is 30% input. The western blot was probed using CBP20 (R21) .“↑”（supernatant）; “↓(deposition）