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TCF-1 (F26) polyclonal antibody（KU）

货号: BS1987

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS1987

Product Name :TCF-1 (F26) polyclonal antibody（KU）

Swiss-Prot :P36402

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IP IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200 IF: 1:100~1:500

Background :T-cell factor-1 (TCF-1) is a DNA-binding transcriptional activator that isessential for lymphoid cell development. The TCF family of transcription factors are activated by the Wnt-1 and Wingless pathways and are characterized by the presence of a conserved protein motif, the high mobility group (HMG) 1 box, which mediates DNA binding. Several alternative splice variants of TCF-1 have been identified, including TCF-1A, which share a conserved amino terminus and differ in the carboxy terminal sequences. The Wnt mediated signaling pathway induces cytosolic β-catenin binding to TCF proteins within the nucleus, leading to the enhanced expression of the Wnt target genes. The β-catenin-TCF complexes are negatively regulated by the adenomatous polyposis coli (APC) tumor suppressor protein, which phosphorylates β-catenin and, in turn, increases the degradation of cytosolic β-catenin and inhibits the transcriptional activity of the TCF proteins.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :TCF-1 (F26) polyclonal antibody detects endogenous levels of TCF-1 protein.

BiowMW :~ 42 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Wntβ catenin signaling，

Alternative Name :Transcription factor 7; TCF-7; T-cell-specific transcription factor 1; T-cell factor 1; TCF-1; TCF7; TCF1

Immunogen :Synthetic peptide, corresponding to amino acids N-terminus of Human TCF-1.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of TCF-1 (F26) pAb at 1:500 dilution Lane1:K562 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:U-87MG whole cell lysate(40ug) Lane4:C6 whole cell lysate(40ug) Lane5:MEF whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of TCF-1 (F26) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Immunofluorescence analysis of L02 cells using TCF-1 (F26) pAb at dilution of 1:200 (40x lens).

Immunoprecipitation of HCT116 cell lysates using TCF-1 pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using TCF-1 pAb.