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CstF-64 (R46) polyclonal antibody（KU）

货号: BS1980

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS1980

Product Name :CstF-64 (R46) polyclonal antibody（KU）

Swiss-Prot :P33240

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :CstF-64 contains an RNA binding domain and is responsible for the RNA binding activity of CstF. CstF-64 is expressed in all somatic cells and in pre- and postmeiotic, but not meiotic, germ cells. However, a large variant of CstF-64, called t CstF-64, is abundantly expressed in meiotic and postmeiotic cells in the testis and to a lesser extent in the brain, and promotes the germ cell pattern of polyadenylation. The gene encoding CstF-64 (designated CSTF2) maps to the X chromosome, whereas t CstF-64 is encoded by an autosomal gene. The increase in CstF-64 concentration during B cell activation switches IgM heavy chain mRNA expression from membrane-bound to secreted forms, suggesting that CstF-64 plays a key role in regulating IgM heavy chain expression during B cell differentiation.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :CstF-64 (R46) polyclonal antibody detects endogenous levels of CstF-64 protein.

BiowMW :~ 64 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Cleavage stimulation factor 64 kDa subunit; CSTF 64 kDa subunit; CF-1 64 kDa subunit; CstF64; CstF 64; CSTF2;

Immunogen :Synthetic peptide, corresponding to amino acids N-terminus of Human CstF-64.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of CstF-64 (R46) pAb at 1:1000 dilution Lane1:K562 whole cell lysate(20ug) Lane2:HEK293T whole cell lysate(20ug) Lane3:C6 whole cell lysate(40ug) Lane4:SP2/0 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of CstF-64 (R46) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.