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NF-YB (Q30) polyclonal antibody（KU）

货号: BS1964

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1964

Product Name :NF-YB (Q30) polyclonal antibody（KU）

Swiss-Prot :P25208

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :The CCAAT-binding factor NF-Y is a heteromeric transcription factor that specifically binds to CCAAT sequences in many eukaryotic genes. NF-Y is made up of three subunits, NF-YA, NF-YB and NF-YC. All three components are necessary for DNA binding. In each NF-Y subunit, the segment needed for formation of the NF-Y-DNA complex is conserved from yeast to human. These conserved segments are homologous to the histone-fold motif of eukaryotic histones. The DNA-binding domains of the NF-YB and NF-YC subunits have been suggested to interact through a protein-protein, histone-fold “handshake” motif in a manner analogous to the histone proteins H2B and H2A, respectively.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :NF-YB (Q30) polyclonal antibody detects endogenous levels of NF-YB protein.

BiowMW :~ 32 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Nuclear transcription factor Y subunit beta; CAAT box DNA-binding protein subunit B; Nuclear transcription factor Y subunit B; NF-YB; NFYB; HAP3

Immunogen :Synthetic peptide, corresponding to the N-terminal of Human NF-YB.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of NF-YB (Q30) pAb at 1:500 dilution Lane1:The Brain tissue lysate of Rat(40ug) Lane2:SGC7901 whole cell lysate(40ug) Lane3:A375 whole cell lysate(40ug) Lane4:Panc1 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of NF-YB (Q30) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.