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UBF-1 (E536) polyclonal antibody(KU)

货号: BS1939
价格 1140
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS1939
Product Name :UBF-1 (E536) polyclonal antibody(KU)
Swiss-Prot :P17480
Host :Rabbit
Reactivity :Human,Mouse,Rat
Applications :WB IHC
Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200
Background :The transcription of ribosomal RNA genes by RNA polymerase I (Pol I) is tightly coordinated with the growth state of the cell. In addition to Pol I, transcription of ribosomal genes requires the trans-activating factor UBF (upstream binding factor). UBF functions by binding to DNA elements within the RNA gene promoter and enhancer regions and directly associating with Pol I, tethering it to the promoter complex. Two UBF proteins, of 97 kDa and 94 kDa, arise from the same gene as a result of alternative mRNA splicings. UBF activity is regulated by several dependent casein kinase II phosphorylates at the carboxy terminus of UBF on serine residues. The retinoblastoma susceptibility gene product, Rb, when not bound to E2F family members, inhibits UBF activity. Expression of RNA may also be negatively regulated by the 70 kDa and 86 kDa Ku antigens.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :UBF-1 (E536) polyclonal antibody detects endogenous levels of UBF-1 protein.
BiowMW :~ 90 kDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :Nucleolar transcription factor 1; Autoantigen NOR-90; Upstream-binding factor 1; UBF-1; UBTF; UBF; UBF1
Immunogen :Synthetic peptide, corresponding to amino acids 500-550 of Human UBF-1.
Conjugate :Unconjugated
Modification :Unmodification
Western blot (WB) analysis of UBF-1 (E536) pAb at 1:500 dilution Lane1:L02 whole cell lysate(40ug) Lane2:H9C2 whole cell lysate(20ug) Lane3:AML-12 whole cell lysate(20ug) Lane4:A549 whole cell lysate(20ug)
Immunohistochemistry (IHC) analyzes of UBF-1 (E536) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com