产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

PKA IIα reg (P68) polyclonal antibody（KU）

货号: BS1929

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1929

Product Name :PKA IIα reg (P68) polyclonal antibody（KU）

Swiss-Prot :P13861

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Cα, Cβ and Cγ, that each represent specific gene products. Cα and Cβ are closely related (93% amino acid sequence similarity), where as Cγ displays 83% and 79% similarity to Cα and Cβ, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PKA IIα reg (P68) polyclonal antibody detects endogenous levels of PKA IIα reg protein.

BiowMW :~ 50 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Inhibition of Apoptosis,Mitochondrial Control of Apoptosis,

Alternative Name :cAMP-dependent protein kinase type II-alpha regulatory subunit; PRKAR2A; PKR2; PRKAR2

Immunogen :Synthetic peptide, corresponding to amino acids 50-100 of Human PKA IIα reg.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of PKA IIα reg (P68) pAb at 1:500 dilution Lane1:PC3 whole cell lysate(10ug) Lane2:U-87MG whole cell lysate(10ug) Lane3:The Uterus tissue lysate of Rat(40ug) Lane4:AML-12 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of PKA IIα reg (P68) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Lipopolysaccharide challenge significantly influences lipid metabolism and proteome of white adipose tissue in growing pigs

JournalLipids in Health and Disease

ApplicationWB

IF3.876

SpeciesPig

PMID