产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

Aldolase A (T9) polyclonal antibody（KU）

货号: BS1912

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1912

Product Name :Aldolase A (T9) polyclonal antibody（KU）

Swiss-Prot :P04075

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :Fructose 1,6-bisphosphate aldolase catalyses the reversible condensation of glycerone-P and glyceraldehyde 3-phosphate into fructose 1,6-bisphosphate. Fructose 1,6-bisphosphate aldolase exists as three forms: the muscle-specific Aldolase A, the liver-specific Aldolase B, and the brain-specific Aldolase C. Aldolase A, B and C arose from a common ancestral gene from which Aldolase B first diverged. Aldolase A is one of the most highly-conserved enzymes known, with only about 2% of the residues changing per 100 million years. Aldolase B is regulated by the hormones insulin and glucagon, and has been implicated in hereditary fructose intolerance disease. Aldolase C is a polypeptide that is exclusively expressed in Purkinje cells. Aldolase C-positive Purkinje cells are organized in the cerebellum as stripes or bands that run from anterior to posterior across the cerebellum and alternate with bands of Aldolase Cnegative Purkinje cells.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Aldolase A (T9) polyclonal antibody detects endogenous levels of Aldolase A protein.

BiowMW :~ 40 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Warburg Effect,

Alternative Name :Fructose-bisphosphate aldolase A; Lung cancer antigen NY-LU-1; Muscle-type aldolase; ALDOA; ALDA

Immunogen :Synthetic peptide, corresponding to the N-terminus of Human Aldolase A.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Aldolase A (T9) pAb at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:U-87MG whole cell lysate(40ug) Lane3:AML-12 whole cell lysate(40ug) Lane4:PC3 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of Aldolase A (T9) pAb in paraffin-embedded human lung carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.