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p27 Kip1 (P191) polyclonal antibody（KU）

货号: BS1858

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS1858

Product Name :p27 Kip1 (P191) polyclonal antibody（KU）

Swiss-Prot :P46527

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Cell cycle progression is regulated by a series of cyclin-dependent kinases that consist of catalytic subunits, designated Cdks, and activating subunits, designated cyclins. Orderly progression through the cell cycle requires the activation and inactivation of different cyclin-Cdks at appropriate times. A series of proteins has been recently described that function as “mitotic inhibitors.” These include p21, the levels of which are elevated upon DNA damage in G1 in a p53-dependent manner, p16 and a more recently described p16 related inhibitor designated p15. A p21 related protein, p27, has been described as a negative regulator of G1 progression and has been speculated to function as a possible mediator of TGFβ-induced G1 arrest. p27 interacts strongly with D-type cyclins and Cdk4 in vitro and to a lesser extent with cyclin E and Cdk2.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p27 Kip1 (P191) polyclonal antibody detects endogenous levels of p27 Kip1 protein.

BiowMW :~ 27 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Cell Cycle Control G1 S Checkpoint,

Alternative Name :Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor; p27; p27Kip1; CDKN1B; KIP1

Immunogen :Synthetic peptide, corresponding to amino acids 170-220 of Human p27 Kip1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of p27 Kip1 (P191) pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:Hela whole cell lysate(40ug) Lane3:The Lung tissue lysate of Rat(40ug) Lane4:AML-12 whole cell lysate(40ug)

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