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MSK1 (Q577) polyclonal antibody（KU）

货号: BS1824

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS1824

Product Name :MSK1 (Q577) polyclonal antibody（KU）

Swiss-Prot :O75582

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :MSK-1 is a mitogen and stress activated protein kinase-1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK-1can be activated by ERK1/2 and SAPK2 /p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG-14 in response to mitogen and stress. Similar to RSK, MSK-1 contains two kinase domains (N-term and a C-term). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK-1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C-terminal kinase domain of MSK-1 which is essential for the catalytic activity of the N-terminal kinase domain.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :MSK1 (Q577) polyclonal antibody detects endogenous levels of MSK1 protein.

BiowMW :~ 90 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Signaling Pathways Activating p38 MAP Kinase,Epigenetic Writers and Erasers of Histone H3,Epigenetic Writers and Erasers of Histones H2A H2B and H4,

Alternative Name :Ribosomal protein S6 kinase alpha-5; S6K-alpha-5; 90 kDa ribosomal protein S6 kinase 5; Nuclear mitogen- and stress-activated protein kinase 1; RSK-like protein kinase; RSKL; RPS6KA5; MSK1

Immunogen :Synthetic peptide, corresponding to amino acids 551-600 of Human MSK1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of MSK1 (Q577) pAb at 1:500 dilution Lane1:C6 whole cell lysate(40ug) Lane2:AML-12 whole cell lysate(40ug) Lane3:A2780 whole cell lysate(40ug) Lane4:HEK293T whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of MSK1 (Q577) pAb in paraffin-embedded human breast carcinoma tissue at 1:50. showing Nucleus and Cytoplasm staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of MSK1 (Q577) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing Nucleus and Cytoplasm staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Interferon regulatory factor 3 is a negative regulator of pathological cardiac hypertrophy

JournalBasic Research In Cardiology

ApplicationWB

IF11.981

SpeciesMouse

PMID